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fab2125g (R&D Systems)

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R&D Systems fab2125g
Fab2125g, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lyve+1/Mouse+LYVE-1+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pm41964950-511-160-157
Average 93 stars, based on 20 article reviews

fab2125g - by Bioz Stars, 2026-10

93/100 stars

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Article Title: AXL promotes lymphangiogenesis by amplifying VEGF-C-mediated AKT pathway.
Article Snippet: After 28 days (=metastatic condition), LNs were collected and either embedded in tissue Tissue-Tek OCT (VWR International) and frozen at − 80 °C or incubated in 4% formol (VWR International) for 4 h, dehydrated in ethanol and fixed in paraffin (Leica Biosystems).

Incubation:

Article Title: AXL promotes lymphangiogenesis by amplifying VEGF-C-mediated AKT pathway
Article Snippet: Then, both type sections were permeabilized for 5 min with 1% Triton X-100 (Millipore) at room temperature and blocked for 20 min in Animal-Free blocking solution (Cell signaling). .. Paraffin sections were incubated with LYVE-1 (R&D Systems Cat# AF2125, RRID: AB_2297188) antibody for 1 h at room temperature. .. After washing with PBS, anti-goat Alexa Fluor 488 antibody (Thermo Fisher Scientific Cat# A-11055 (also A11055), RRID: AB_2534102, 1/200) was added for 30 min at room temperature.

Staining:

Article Title: Rescue of the endogenous FVIII expression in hemophilia A mice using CRISPR Cas9 mRNA LNPs
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Rescue of the endogenous FVIII expression in hemophilia A mice using CRISPR-Cas9 mRNA LNPs
Article Snippet: Mouse livers were harvested, fixed in 4% paraformaldehyde (RT-15710, Electron Microscopy Sciences, PA, USA) for 1 h at room temperature and cryoprotected in PBS (phosphate-buffered saline) with 30% sucrose at 4°C overnight. .. Liver sections were blocked in 0.5% fetal bovine serum in PBS and stained with anti-GFP (A10262, Thermo Scientific, WA, USA), CD31 (14-0311-81, Thermo Scientific) and LYVE-1 (AF2125, R&D Systems, MN, USA) at 4°C overnight, followed by application of the secondary antibodies conjugated with Alexa Fluor 488 or 594 (Invitrogen). .. For vascular staining, PBS containing 20 μg/mL Isolectin GS-IB4 (I21411, Thermo Scientific) with Alexa Fluor 488 conjugate was added to sections and stained at room temperature for 2 h. Sections were mounted with mounting medium containing DAPI (H-1200-10, Vector Laboratories, CA, USA) for nuclear staining.